![]() ![]() Have screened for differentially expressed genes (DEGs) orĭifferentially expressed miRNAs during MSC differentiation into ![]() Profiling using DNA microarrays on MSCs at different time pointsĭuring chondrogenic differentiation. Mrugala et al ( 15) performed large-scale expression De Luca etĮpidermal growth factor receptor signaling affected the secretome MSCs secreted microparticles enriched in pre-miRNAs. Various studies have examined theĭifferential expression of genes or microRNAs (miRNAs) during MSCĭifferentiation using bioinformatics. Have been successful, overall treatment outcomes remain Previous studies have demonstrated that although certain treatments Liposarcoma, put tremendous burden on patients worldwide ( 8– 10). Osteoblast- and adipocyte-associated diseases, suchĪs bone disorders, cartilage injury, bone damage, angiopathy and Pathways, including mitogen-activated protein kinase, Wnt, Notch Osteoblastic or adipocytic differentiation occurs when MSCs areĬultured in three conditions in vitro: i) Three-dimensionalĬulture format ii) serum-free nutrient medium and iii) with theĪddition of inducible factors, such as bone morphogenetic protein 6īMP-6 or dexamethasone may induce osteoblastic or adipocyticĭifferentiation of MSCs, respectively, via crosstalk with signaling Most promising seed cells for the treatment of osteoblastic or To differentiate into numerous cell types ( 1). Mesenchymal stem cells (MSCs) were discovered inīone marrow and are multipotent stromal cells that have the ability The results of the present study suggested that miRNAs (miR‑203 and miR‑382) and DEGs (NEGR1, PPAP2B, PDGFRA, IL6ST and SORT1) may serve pivotal functions in the osteoblastic differentiation of MSCs, whereas miR‑495, which is also involved in osteoblast differentiation and had four targets, including NEGR1, miR‑376a, miR‑543 and ENAH may have crucial roles in adipocytic differentiation of MSCs. In addition, the downregulated miRNAs (including miR‑495, miR‑376a and miR‑543), the upregulated miR‑106a, the upregulated DEGs, including enabled homolog (ENAH), polypeptide N‑acetylgalactosaminyltransferase 1 and acyl‑CoA synthetase long‑chain family member 1, and the downregulated repulsive guidance molecule family member B and semaphorin SEMA7A were demonstrated to be involved in adipocytic differentiation. miRNAs, including miRNA (miR)‑382 and miR‑203, and DEGs, including neuronal growth regulator 1 (NEGR1), phosphatidic acid phosphatase 2B (PPAP2B), platelet‑derived growth factor receptor alpha (PDGFRA), interleukin 6 signal transducer (IL6ST) and sortilin 1 (SORT1), were demonstrated to be involved in osteoblastic differentiation. Important pathways, such as glutathione metabolism, pathogenic Escherichia coli infection and Parkinson's disease, and GO terms, including cytoskeletal protein binding and phospholipase inhibitor activity, were enriched in the screened DEGs from MSCs undergoing osteogenic differentiation and adipocytic differentiation. Numerous DEGs and miRNAs were screened during osteoblastic and adipocytic differentiation of MSCs. In addition, an interaction network between the DEGs and miRNAs was constructed. Subsequent gene ontology (GO) and pathway analyses on the DEGs were performed using the GO and Kyoto Encyclopedia of Genes and Genomes databases, respectively potential target genes for the screened miRNAs were predicted using the TargetScan database. The t‑test in the Bioconductor bioinformatics software tool was used to screen DEGs and differentially expressed miRNAs in the two samples. Bone morphogenetic protein 6 (BMP‑6) and dexamethasone were used to induce MSCs towards osteoblastic differentiation or adipocytic differentiation. The present study aimed to screen several differentially expressed genes (DEGs) and differentially expressed microRNAs (miRNAs) for two types of mesenchymal stem cell (MSC) differentiation. ![]()
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